Biologically Active Substances of Protozoa by N.N. Sukhareva-Buell

By N.N. Sukhareva-Buell

The look for new manufacturers of biologically energetic ingredients (BAS) opposed to human and animal ailments remains to be an incredible job in biology and medication. Experimental paintings has to be conducted good ahead of want since it takes a standard of ten years to increase a brand new drugs, in addition to time beyond regulation to place it out there. research of the Protozoa types a distinct department of biology - protozoology. the normal fields of protozoology are taxonomy, phylogeny, morphology, cytology, evolution, ecology and host parasite-interactions. The Protozoa is the single taxon one of the microscopic organisms, which has now not been again and again studied as a resource of BAS. This booklet then is the results of the study at the venture: "Biologically lively ingredients of the Mastigophora (Flagellates)". The study was once performed on the Laboratory of Antibiotics, division of Microbiology, organic school of Moscow country collage. Articles of alternative authors at the subject were regarded as the real a part of this reference publication. The target of the reference e-book is to clarify clinical ways, which bring about acquiring biologically energetic ingredients from cultures of protozoa; the e-book experiences the old historical past in reference to modern improvement of the sector. N.N. Sukhareva ACKNOWLEDGMENTS The learn was once played in fruitful cooperation with my learn affiliates (V. Urinyuk, T. Titiova, L. Udalova, R. Zeleneva, V. Brusovanik, M. Zaretskaya), postgraduate scholars (N. Kalenik, M. Chuenkova, V. Vasilevskaya, V. Khorokhorina), my colleagues at Moscow country collage (Yu. Kozlov and I.

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1987). Phospholipid content is rather high in flagellates and can reach 50-60% of the total lipid fraction. Phospholipid fractions of zooflagellates are characterized by a high content of phosphatidylcholine (PC-up to 60-80% of the total phopholipids) and phosphatidylethanolamine (PE-15-40%), and lower amounts of sphingomyeline (3-10%), phosphatidylserine (3-5%), phosphatidylinositol (1-3%) and phosphatidic acids (9-10%). PC and PE have been found in acetyl and plasmalogen forms in Zoojlajlagellates (Urinyuk and Sukhareva, 1972; Sukhareva, 1989).

Free-living flagellates such as A. /onga and lower trypanosomatids such as C. oncopelti don't need additives for the protection of their cell membranes . 5 volume of air per one liter of a medium. Bubble coalescence and impeller use are not allowed. Sparged aeration plays two roles: it supplies cultures with air (or other gases mixed with air) and maintains cells in suspension. The recommended maximum volume of a bioreactor is 500 liters, filled with 200 liters of a medium. Energy expenditures for one operation in a bioreactor during 5-7 days of cultivation are approximately 250-300 kwt.

1999), Their primary role is associated with the protection of polyunsaturated lipids from the free radicals that cause lipid oxidation in membranes (Vladimirov and Archakov, 1972; Wang and Quinn, 2000). Vitamin E plays an important role in stabilizing membranes through the formation of a-tocopherol and long chain polyunsaturated fatty acid, or Iysophospholipid, complexes (Wang and Quinn, 2000). Lipid-soluble ubiquinones increase their antioxidant effect through elongation of the side chain, from QI to QIO (Bliznakov, 1976) Cultures of C.

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